ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (12): 2192-2198.doi: 10.11843/j.issn.0366-6964.2015.12.010

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The Effect of IFN-γ on Melanocytes Proliferation and Melanogenesis of Alpaca Skin

ZHAO Yi-wen1,ZHAO Li-jun1,2,LI Zhen1,YIN Zhi-hong1,BAI Rui1,ZHAO Min3,PANG Quan-hai1*   

  1. (1.College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801, China;2.Henan Hualong Biological Technology Limited Company,Xinxiang 453000,China;3.Institute of Animal Health Supervision of Shaanxi Province,Xi’an 710016,China)
  • Received:2015-01-21 Online:2015-12-23 Published:2015-12-23

Abstract:

The effect of IFN-γ on melanocytes proliferation and melanogenesis was studied.Different concentrations of IFN-γ (0.0,0.5,2.0,8.0,16.0,32.0,64.0 ng•mL-1) treated separately melanocytes of Alpaca skin at 24,48,72 h.The activity of melanocytes was detected using MTT method,the melanin content was tested with ultraviolet spectrophotometric method and qRT-PCR was used to detect the changes of MC1R,TYRTYRP1,MITFDCT and NOS2 on mRNA level.The results showed that the melanocytes activity was inhibited after 24 h,melanocytes activity recovered and the melanogenesis was enhanced after 72 h.IFN-γ promoted the increase of melanin content and 32.0 ng•mL-1 IFN-γ increased significantly.The relative mRNA expression increased significantly on TYRP1,MITFDCTNOS2 (P<0.05),but there were no significant differences on MC1R,TYR (P>0.05).IFN-γ promoted the growth of melanocyte dendrites,which may contribute to melanin transport;IFN-γ induced melanogenesis,which may be related to NOS2 and MITF high expression in melanoma cells and the melanin may be produced through NO/cGMP/PKG-mediated signal pathway.

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